Restriction Enzymes
New England Biolabs, Inc. BseYI – 500 units
A restriction endonuclease that recognizes the sequence C^CCAG_C.
New England Biolabs, Inc. SfoI – 500 units
A restriction endonuclease that recognizes the sequence GGC_^GCC.
New England Biolabs, Inc. Nt.BstNBI – 1000 units
A restriction endonuclease that recognizes the sequence GAGTCNNNN^N.
New England Biolabs, Inc. BsaXI – 500 units
A restriction endonuclease that recognizes the sequence _NNN^NNNNNNNNNACNNNNNCTCCNNNNNNN_NNN^.
New England Biolabs, Inc. BaeI – 250 units
A restriction endonuclease that recognizes the sequence _NNNNN^NNNNNNNNNNACNNNNGTAYCNNNNNNN_NNNNN^.
New England Biolabs, Inc. RsaI – 1000 units
A restriction endonuclease that recognizes the sequence GT_^AC.
AURORA BIOLABS LLC Human Malic enzyme 2 (ME2), 100 μg
Mitochondrial NAD-dependent malic enzyme (ME2) is a homotetrameric protein that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene.
New England Biolabs, Inc. MfeI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence C^AATT_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. PI-SceI – 250 units
A restriction endonuclease that recognizes the sequence ATCTATGTCGG_GTGC^GGAGAAAGAGGTAAT. The intein encoding PI-SceI is present in the VMA ATPase gene Saccharomyces cerevisiae. The gene has been modified for independent expression in E.coli using a T7 RNA polymerase expression system.
New England Biolabs, Inc. BmtI-HF – 1500 units
A high fidelity restriction endonuclease that recognizes the sequence G_CTAG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. SpeI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence A^CTAGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. EcoGII Methyltransferase – 200 units
EcoGII Methyltransferase is a non-specific methyltransferase that modifies adenine residues (N6) in any sequence context.
New England Biolabs, Inc. Endo H – 10000 units
Endoglycosidase H is a recombinant glycosidase which cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins.
New England Biolabs, Inc. HaeIII – 3000 units
A restriction endonuclease that recognizes the sequence GG_^CC.
New England Biolabs, Inc. BssHII (25,000 units/ml) – 2500 units
A restriction endonuclease that recognizes the sequence G^CGCG_C.